Arch Biochem Biophys 196: 301–303, Reichhart D, Salaiin J-P, Benveniste I, Durst F (1980) Induction of cytochrome P-450, NADPH cytochrome c reductase and cinnamic acid 4-hydroxylase by manganese, ethanol, phenobarbital and herbicides in higher plants. Purification and immunocharacterization of a plant cytochrome-P450—the cinnamic acid 4-hydroxylase. In: Tevini M, Lichtenthaler HK (eds) Lipids and lipid polymers in higher plants. FEBS Lett 188: 11–14, Petersen M, Alfermann AW, Reinhard E, Seitz HU (1987) Immobilisation of digitoxin 12a-hydroxylase, a cytochrome P-450-dependent enzyme from cell cultures of Digitalis Ianata EHRH. The number in parenthesis next to the species name is the total P450 count in the species. The present status of plant cytochrome P450 research is reviewed. Over 10 million scientific documents at your fingertips. FEBS Lett 169: 7–11, Hagmann M-L, Grisebach H (1984) Enzymatic rearrangement of flavanone to isoflavone. Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Plant Physiol 70: 573–578, Gabriac B, Werck-Reichhart D, Teutsch H, Durst F (1991) Purification and immunocharacterization of a plant cytochrome P450: the cinnamic acid 4-hydroxylase. J-STAGE, Japan Science and Technology Information Aggregator, Electronic. Members of this superfamily are involved in multiple metabolic pathways with distinct and complex functions, playing important roles in a vast array of reactions. The rapidly advancing characterization of new cytochrome P450 enzymes in plants (Durst 1991) shows that cytochromes P450 are involved in the biosynthetic pathway of major phytoalexins (chemicals synthesized by plants to deter hostile organisms). If this is true, plants should provide a vast reservoir of cytochrome P450 genes.  |  Pestic Biochem Physiol 34: 92–100, McFadden JJ, Gronwald JW, Eberlein CV (1990) In vitro hydroxylation of bentazon by microsomes from naphthalic anhydride-treated corn shoots. Some essential P450 functions are conserved among plant species, including hormone, sterol and oxygenated fatty acid synthesis. This implies that the diversification of P450 has made a significant contribution to the ability to acquire the emergence of new metabolic pathways during land plant evolution. In plants and animals, CYPs play a central role in the detoxification of xenobiotics. Plant Physiol 80: 483–486, Madyastha KM, Ridgway JE, Dwyer JG, Coscia CJ (1977) Subcellular localization of a cytochrome P450 dependent monooxygenase in vesicles of the higher plant Catharanthus roseus. Biochemical investigations This service is more advanced with JavaScript available, Cytochrome P450 Cytochrome P450s (CYPs) are the largest enzyme family involved in NADPH- and/or O 2 -dependent hydroxylation reactions across all the domains of life. Biochem Biophys Res Commun 53: 1043–1048, Moreland DE, Corbin FT, Novitzky WP (1990) Metabolism of metolachlor by a microsomal fraction isolated from grain sorghum ( Sorghum bicolor) shoots. Proc Natl Acad Sci USA 85: 7221–7225, Karp F, Mihalaik CA, Harris JL, Croteau R (1990) Monoterpene biosynthesis: specificity of the hydroxylations of (-)-limonene by enzyme preparations from peppermint (Mentha piperita), spermint (Mentha spicata) and perilla ( Perilla frutescens) leaves. Biochem Biophys Res Commun 184: 183–193, Potts JM, Weklych R, Conn EC (1974) The 4-hydroxylation of cinnamic acid by sorghum microsomes and the requirement for cytochrome P-450. Photochem Photobiol 42: 779–783, Kochs G, Grisebach H (1986) Enzymic synthesis of isoflavones. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Plant Physiol 85: 457–462, Kalb VF, Loper JC (1988) Proteins from eight eukaryotic cytochrome P-450 families share a segmented region of sequence similarity. Phytochemistry 17: 359–363, Benveniste I, Salaun J-P, Simon A, Reichhart D, Durst F (1982) Cytochrome P-450 dependent ω-hydroxylation of lauric acid by microsomes from pea seedlings. Metabolic engineering with plant P450s is an important technology for large-scale production of valuable phytochemicals such as medicines. Plant Sci Lett 22: 39–46, Benveniste I, Durst F (1974) Mise en évidence dans les tissus du Tubercule de Topinambour (Helianthus tuberosus L.) d’une enzyme à cytochrome P-450, l’acide tr-cinnamique 4-hydroxylase. Phytochemistry 15: 379–385, Zimmerlin A, Durst F (1990) Xenobiotic metabolism in plants: aryl hydroxylation of diclofop by a cytochrome P-450 enzyme from wheat. Psoralen 5- monooxygenase activity from elicitor-treated Ammi majus cells. Science 253: 781–784, Stewart CB, Schuler MA (1989) Antigenic crossreactivity between bacterial and plant cytochrome P-450 monooxygenases. FEBS Lett 246: 120–126, Saunders JA, Conn EE, Lin CL, Shimada M (1977) Localization of cinnamic acid 4-hydroxylase and the membrane-bound enzyme system for dhurrin biosynthesis in sorghum seedlings. The capacity of coi1 plants to produce significant amounts of 12OH-JA-Ile , however, indicates the existence of a COI1-independent route for 12OH-JA-Ile formation. Genes Genomics. In: Stumpf RK, Conn EE (eds) The biochemistry of plants, vol 2. Biochem Biophys Res Commun 140: 1064–1072, Reichhart D, Salaiin J-P, Benveniste I, Durst F (1979) Induction by manganese, ethanol, phenobarbital and herbicides of microsomal cytochrome P-450 in higher plant tissues. Academic, New York, Young O, Beevers H (1976) Mixed function oxidases from germinating castor bean endosperm. Phytochemistry 29: 1113–1122, Tanaka Y, Kojima M, Uritani I (1974) Properties, development and cellular localization of cinnamic acid 4-hydroxylase in cut-injured sweet potato. Biochem Biophys Res Commun 168: 206–213, Meehan TD, Coscia CJ (1973) Hydroxylation of geraniol and nerol by a monoxygenase from Vinca rosea. Eur J Biochem 155: 311–318, Kochs G, Grisebach H (1987) Induction and characterization of a NADPH-dependent flavone synthase from cell cultures of soybean. Eur J Biochem 171: 369–375, Hamerski D, Matern U (1988b) Biosynthesis of psoralens. The cytochrome P450 (CYP) superfamily is the largest enzymatic protein family in plants, and it also widely exists in mammals, fungi, bacteria, insects and so on. Front Plant Sci. To assess if the target site alterations bestow resistance, the ALS gene, the molecular target of chlorsulfuron, was sequenced from GL-1. PubMed Google Scholar. Phytochemistry 29: 1729–1732, https://doi.org/10.1007/978-3-642-77763-9_19. HHS Diverse roles of cytochrome P450s in plants. A driving force for the adoption of integrated weed management. Cite as. Cytochrome P450s (CYPs) are the largest enzyme family involved in NADPH- and/or O 2 -dependent hydroxylation reactions across all the domains of life. We describe the design of a series of artificial isoflavone … This study paves the way for engineering the production of diosgenin and derived analogs in heterologous hosts. eCollection 2020. Plants typically devote about 1% of the protein-coding genes for the P450s to execute primary metabolism and also to perform species-specific specialized functions including metabolism of the triterpenes, isoprene-derived 30-carbon compounds. Annu Rev Plant Biol. Despite this, several P450 coding sequences from plant sources are discovered yearly but only a few have been screened by functional genomics. 2021 Jan;43(1):11-16. doi: 10.1007/s13258-020-01011-w. Epub 2020 Nov 10. Eur J Biochem 72: 353–360, Salaün J-P, Benveniste I, Reichhart D, Durst F (1978) A microsomal cytochrome P450-linked lauric acid monooxygenase from aged Jerusalem artichoke tuber tissues. In addition, although a large number of P450 genes have been obtained in different insect species, the number of P450s in different insects varies considerably, for instance, there are 100 and 80 P450 genes in C. quinquefasciatus (Yang and Liu, 2011), 64 P450 genes in Acyrthosiphon pisum (Zhang et al., 2010), and only 36 P450 genes in Pediculus humans (Lee et al., 2010). Biochem J 270: 729–735, West CA (1980) Hydroxylases, monooxygenases and cytochrome P-450. Pestic Biochem Physiol 37: 165–173, Frear DS, Swanson HR, Tanaka FS (1969) N-Demethylation of substituted 3- (phenyl)l-methylureas: isolation and characterization of a microsomal mixed function oxidase from cotton. Although many CYP86 and CYP94 have been characterized biochemically, their physiological function in a biological process is mostly unknown. Annu Rev Plant Physiol 28: 479–501, Tanahashi T, Zenk MH (1990) Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis. Agric Biol Chem 45: 1911–1913, Fujita M, Oba K, Uritani I (1982) Properties of a mixed function oxygenase catalysing ipomeamarone 15-hydroxylation in microsomes from cut-injured and Ceratocystis fimbriata-infected sweet potato root tissues. The metabolism of xenobiotics has mainly been investigated in higher plant species. Cytochrome P450 monooxygenases play a significant role in the detoxification of hostplant allelochemicals and synthetic insecticides in Lepidoptera. Pestic Biochem Physiol 30: 178–189, Wendorf H, Matern U (1986) Differential response of cultured parsley cells to elicitors from two non-pathogenic strains of fungi microsomal conversion of (+)marmesin into psoralen. A survey of the largest family of genes coding for metabolic enzymes, cytochromes P450 (P450s), on eight land plant genomes showed that most P450 families with essential housekeeping functions, involved for example in the biosynthesis of lignin precursors or hormone homeostasis, are present in low-copy, sometimes single-copy number, and broadly distributed across plant taxa (Nelson and Werck- … One may hypothesize that pressure from this coevolutionary process has driven cytochrome P450 in opposite directions: the evolution of phytoalexin-metabolizing forms with broad and overlapping substrate specificity in animals, and the evolution of specific isoforms that participate in the synthesis of these phytoalexins in plants. Eur J Biochem 159: 163–169, Bozak KR, Yu H, Sirevåg R, Christoffersen RE (1990) Sequence analysis of ripening-related cytochrome P-450 cDNAs from avocado fruit. Cytochrome P450 (P450) is a hemoprotein which acts as the terminal oxidase in monooxygenase systems. The F1 and F2 progeny were generated by crossing GL-1 with BTx623. 2010 Nov 9;10:243. doi: 10.1186/1471-2229-10-243. Phytochemistry 16: 69–73, Benveniste I, Salaun J-P, Durst F (1978) Phytochrome mediated regulation of a monooxygenase hydroxylating cinnamic acid in etiolated pea seedlings. Cytochrome P450 reductase (EC 1.6.2.4; also known as NADPH:ferrihemoprotein oxidoreductase, NADPH:hemoprotein oxidoreductase, NADPH:P450 oxidoreductase, P450 reductase, POR, CPR, CYPOR) is a membrane-bound enzyme required for electron transfer from NADPH to cytochrome P450 and other heme proteins including heme oxygenase in the endoplasmic reticulum of the eukaryotic cell Z Naturforsch [c] 42: 343–348, Kochs G, Grisebach H (1989) Phytoalexin synthesis in soybean: purification and reconstitution of cytochrome P450 3,9-dihydroxypterocarpan 6a-hydroxylase and of cytochrome P450 cinnamate 4-hydroxylase. 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